publications

2024

PIP2 inhibits pore opening of the cyclic nucleotide-gated channel SthK

Thon O, Wang Z, Schmidpeter PAM, Nimigean CM. Nat Commun. 2024 Sep 19;15(1):8230. doi: 10.1038/s41467-024-52469-1.

Propofol rescues voltage-dependent gating of HCN1 channel epilepsy mutants.

Kim ED, Wu X, Lee S, Tibbs GR, Cunningham KP, Di Zanni E, Perez ME, Goldstein PA, Accardi A, Larsson HP, Nimigean CM. Nature. 2024 Aug;632(8024):451-459. doi: 10.1038/s41586-024-07743-z.

2023

A pentameric TRPV3 channel with a dilated pore

Lansky S, Betancourt JM, Zhang J, Jiang Y, Kim ED, Paknejad N, Nimigean CM, Yuan P, Scheuring S. Nature. 2023 Sep;621(7977):206-214. doi: 10.1038/s41586-023-06470-1.

Calcium-gated potassium channel blockade via membrane-facing fenestrations

Fan C, Flood E, Sukomon N, Agarwal S, Allen TW, Nimigean CM. Nat Chem Biol. 2023 Aug 31. doi: 10.1038/s41589-023-01406-2. Epub ahead of print.

Discrimination between cyclic nucleotides in a cyclic nucleotide-gated ion channel

Pan Y, Pohjolainen E, Schmidpeter PA, Vaiana AC, Nimigean CM, Grubmüller H, Scheuring S. Nat Struct Mol Biol. 2023 Apr;30(4):512-520. doi: 10.1038/s41594-023-00955-3.

Membrane phospholipids control gating of the mechanosensitive potassium leak channel TREK1

Schmidpeter PA, Petroff JT 2nd, Khajoueinejad L, Wague A, Frankfater C, Cheng WW, Nimigean CM, Riegelhaupt PM. Nat Commun. 2023 Feb 25;14(1):1077. doi: 10.1038/s41467-023-36765-w

Ball-and-Chain Inactivation in Potassium Channels

Sukomon N, Fan C, Nimigean CM. Annu Rev Biophys. 2023 May 9;52:91-111. doi: 10.1146/annurev-biophys-100322-072921

2022

Gating intermediates reveal inhibitory role of the voltage sensor in a cyclic nucleotide-modulated ion channel

Gao X, Schmidpeter PA, Berka V, Durham RJ, Fan C, Jayaraman V, Nimigean CM. Nat Commun. 2022 Nov 14;13(1):6919. doi: 10.1038/s41467-022-34673-z

Anionic lipids unlock the gates of select ion channels in the pacemaker family

Schmidpeter PA, Wu D, Rheinberger J, Riegelhaupt PM, Tang H, Robinson CV, Nimigean CM. Nat Struct Mol Biol. 2022 Nov;29(11):1092-1100. doi: 10.1038/s41594-022-00851-2

35 years of channelling potassium ions

Nimigean CM. Nature. 2022 Aug;608(7924):670-672. doi: 10.1038/d41586-022-02163-3

2021

Correlation of membrane protein conformational and functional dynamics

Sanganna Gari RR, Montalvo-Acosta JJ, Heath GR, Jiang Y, Gao X, Nimigean CM, Chipot C, Scheuring S. Nat Commun. 2021 Jul 16;12(1):4363. doi: 10.1038/s41467-021-24660-1

Correlating ion channel structure and function

Schmidpeter PA, Nimigean CM. Methods Enzymol. 2021;652:3-30. doi: 10.1016/bs.mie.2021.02.016

2020

Mechanistic insights into volatile anesthetic modulation of K2P channels

Wague A, Joseph TT, Woll KA, Bu W, Vaidya KA, Bhanu NV, Garcia BA, Nimigean CM, Eckenhoff RG, Riegelhaupt PM.. Elife. 2020 Dec 21;9:e59839. doi: 10.7554/eLife.59839

Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel

Schmidpeter PA, Rheinberger J, Nimigean CM. Nat Commun. 2020 Dec 16;11(1):6401. doi: 10.1038/s41467-020-20104-4

An ACE2 Microbody Containing a Single Immunoglobulin Fc Domain Is a Potent Inhibitor of SARS-CoV-2

Tada T, Fan C, Chen JS, Kaur R, Stapleford KA, Gristick H, Dcosta BM, Wilen CB, Nimigean CM, Landau NR. Cell Rep. 2020 Dec 1:108528. doi: 10.1016/j.celrep.2020.108528

Selectivity filter ion binding affinity determines inactivation in a potassium channel

Boiteux C, Posson DJ, Allen TW, Nimigean CM. Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29968-29978. doi: 10.1073/pnas.2009624117

Time-resolved cryo-EM using Spotiton

Dandey VP, Budell WC, Wei H, Bobe D, Maruthi K, Kopylov M, Eng ET, Kahn PA, Hinshaw JE, Kundu N, Nimigean CM, Fan C, Sukomon N, Darst SA, Saecker RM, Chen J, Malone B, Potter CS, Carragher B. Nat Methods. 2020 Sep;17(9):897-900. doi: 10.1038/s41592-020-0925-6

Polyamine block of MthK potassium channels

Nimigean CM. J Gen Physiol. 2020 Jul 6;152(7):e202012614. doi: 10.1085/jgp.202012614

Ball-and-chain inactivation in a calcium-gated potassium channel

Fan C, Sukomon N, Flood E, Rheinberger J, Allen TW, Nimigean CM. Nature. 2020 Apr;580(7802):288-293. doi: 10.1038/s41586-020-2116-0

Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses

Schmidpeter PA, Sukomon N, Nimigean CM. Methods Mol Biol. 2020;2127:191-205. doi: 10.1007/978-1-0716-0373-4_14

2019

Direct binding of phosphatidylglycerol at specific sites modulates desensitization of a ligand-gated ion channel

Tong A, Petroff JT 2nd, Hsu FF, Schmidpeter PA, Nimigean CM, Sharp L, Brannigan G, Cheng WW. Elife. 2019 Nov 14;8. pii: e50766. doi: 10.7554/eLife.50766

Identifying coupled clusters of allostery participants through chemical shift perturbations

Xu Y, Zhang D, Rogawski R, Nimigean CM, McDermott AE. Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2078-2085. doi: 10.1073/pnas.1811168116

Structural basis of Ca2+-dependent activation and lipid transport by a TMEM16 scramblase

Falzone ME, Rheinberger J, Lee BC, Peyear T, Sasset L, Raczkowski AM, Eng ET, Di Lorenzo A, Andersen OS, Nimigean CM, Accardi A. Elife. 2019 Jan 16;8. pii: e43229. doi: 10.7554/eLife.43229.

2018

Fluorescence Titrations to Determine the Binding Affinity of Cyclic Nucleotides to SthK Ion Channels

Schmidpeter PA, Nimigean CM. Bio Protoc. 2018 Oct 5;8(19). pii: e3041. doi: 10.21769/BioProtoc.3041.

An iris diaphragm mechanism to gate a cyclic nucleotide-gated ion channel

Marchesi A, Gao X, Adaixo R, Rheinberger J, Stahlberg H, Nimigean CM, Scheuring S. Nat Commun. 2018 Sep 28;9(1):3978. doi: 10.1038/s41467-018-06414-8.

Ligand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structures

Rheinberger J, Gao X, Schmidpeter PA, Nimigean CM. Elife. 2018 Jul 20;7. pii: e39775. doi: 10.7554/eLife.39775.

Ligand binding and activation properties of the purified bacterial cyclic nucleotide-gated channel SthK

Schmidpeter PA, Gao X, Uphadyay V, Rheinberger J, Nimigean CM. J Gen Physiol. 2018 Jun 4;150(6):821-834. doi: 10.1085/jgp.201812023.

High-Resolution Cryoelectron Microscopy Structure of the Cyclic Nucleotide-Modulated Potassium Channel MloK1 in a Lipid Bilayer

Kowal J, Biyani N, Chami M, Scherer S, Rzepiela AJ, Baumgartner P, Upadhyay V, Nimigean CM, Stahlberg H. Structure. 2018 Jan 2;26(1):20-27.e3. doi: 10.1016/j.str.2017.11.012.

Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies

Posson DJ, Rusinova R, Andersen OS, Nimigean CM. Methods Mol Biol. 2018;1684:223-235. doi: 10.1007/978-1-4939-7362-0_17.

2017

Mechanism of activation at the selectivity filter of the KcsA K+ channel

Heer FT, Posson DJ, Wojtas-Niziurski W, Nimigean CM, Bernèche S. Elife. 2017 Oct 10;6. pii: e25844. doi: 10.7554/eLife.25844.

Identification of a pre-active conformation of a pentameric channel receptor

Menny A, Lefebvre SN, Schmidpeter PA, Drège E, Fourati Z, Delarue M, Edelstein SJ, Nimigean CM, Joseph D, Corringer PJ. Elife. 2017 Mar 15;6. pii: e23955. doi: 10.7554/eLife.23955.

Real-time visualization of conformational changes within single MloK1 cyclic nucleotide-modulated channels

Rangl M, Miyagi A, Kowal J, Stahlberg H, Nimigean CM, Scheuring S. Nat Commun. 2016 Sep 20;7:12789. doi: 10.1038/ncomms12789.

2016

Conformational heterogeneity in closed and open states of the KcsA potassium channel in lipid bicelles

Kim DM, Dikiy I, Upadhyay V, Posson DJ, Eliezer D, Nimigean CM. J Gen Physiol. 2016 Aug;148(2):119-32. doi: 10.1085/jgp.201611602. Epub 2016 Jul 18.

Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating

Kim DM, Nimigean CM. Cold Spring Harb Perspect Biol. 2016 May 2;8(5). pii: a029231. doi: 10.1101/cshperspect.a029231.

2015

Calcium ions open a selectivity filter gate during activation of the MthK potassium channel

Posson DJ, Rusinova R, Andersen OS, Nimigean CM. Nat Commun. 2015 Sep 23;6:8342. doi: 10.1038/ncomms9342.

2014

Regulation of ion channel function by the host lipid bilayer examined by a stopped-flow spectrofluorometric assay

Rusinova R, Kim DM, Nimigean CM, Andersen OS. Biophys J. 2014 Mar 4;106(5):1070-8. doi: 10.1016/j.bpj.2014.01.027.

A KcsA/MloK1 chimeric ion channel has lipid-dependent ligand-binding energetics

McCoy JG, Rusinova R, Kim DM, Kowal J, Banerjee S, Jaramillo Cartagena A, Thompson AN, Kolmakova-Partensky L, Stahlberg H, Andersen OS, Nimigean CM. J Biol Chem. 2014 Apr 4;289(14):9535-46. doi: 10.1074/jbc.M113.543389.

Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1

Kowal J, Chami M, Baumgartner P, Arheit M, Chiu PL, Rangl M, Scheuring S, Schröder GF, Nimigean CM, Stahlberg H. Nat Commun. 2014;5:3106. doi: 10.1038/ncomms4106.

2013

Molecular interactions involved in proton-dependent gating in KcsA potassium channels

Posson DJ, Thompson AN, McCoy JG, Nimigean CM. J Gen Physiol. 2013 Dec;142(6):613-24. doi: 10.1085/jgp.201311057.

Preparation of uniformly isotope labeled KcsA for solid state NMR: expression, purification, reconstitution into liposomes and functional assay

Bhate MP, Wylie BJ, Thompson A, Tian L, Nimigean CM, McDermott AE. Protein Expr Purif. 2013 Oct;91(2):119-24. doi: 10.1016/j.pep.2013.07.013.

The voltage-dependent gate in MthK potassium channels is located at the selectivity filter

Posson DJ, McCoy JG, Nimigean CM. Nat Struct Mol Biol. 2013 Feb;20(2):159-66. doi: 10.1038/nsmb.2473.

2012

Structural correlates of selectivity and inactivation in potassium channels

McCoy JG, Nimigean CM. Biochim Biophys Acta. 2012 Feb;1818(2):272-85. doi: 10.1016/j.bbamem.2011.09.007.

2011

Origins of ion selectivity in potassium channels from the perspective of channel block

Nimigean CM, Allen TW. J Gen Physiol. 2011 May;137(5):405-13. doi: 10.1085/jgp.201010551.

Mechanism for selectivity-inactivation coupling in KcsA potassium channels

Cheng WW, McCoy JG, Thompson AN, Nichols CG, Nimigean CM. Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5272-7. doi: 10.1073/pnas.1014186108.

Non-vesicular transfer of membrane proteins from nanoparticles to lipid bilayers

Banerjee S, Nimigean CM. J Gen Physiol. 2011 Feb;137(2):217-23. doi: 10.1085/jgp.201010558.

2010

Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB

Tanabe M, Nimigean CM, Iverson TM. Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6811-6. doi: 10.1073/pnas.0912115107.

2009

Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore

Thompson AN, Kim I, Panosian TD, Iverson TM, Allen TW, Nimigean CM. Nat Struct Mol Biol. 2009 Dec;16(12):1317-24. doi: 10.1038/nsmb.1703

2008

Molecular mechanism of pH sensing in KcsA potassium channels

Thompson AN, Posson DJ, Parsa PV, Nimigean CM. Proc Natl Acad Sci U S A. 2008 May 13;105(19):6900-5. doi: 10.1073/pnas.0800873105

2007

The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 A resolution

Chiu PL, Pagel MD, Evans J, Chou HT, Zeng X, Gipson B, Stahlberg H, Nimigean CM. Structure. 2007 Sep;15(9):1053-64. doi: 10.1016/j.str.2007.06.020

Ligand binding and activation in a prokaryotic cyclic nucleotide-modulated channel

Nimigean CM, Pagel MD. J Mol Biol. 2007 Aug 31;371(5):1325-37. doi: 10.1016/j.jmb.2007.06.030

2006

A radioactive uptake assay to measure ion transport across ion channel-containing liposomes

Nimigean CM. Nat Protoc. 2006;1(3):1207-12. doi: 10.1038/nprot.2006.166

Calcium-dependent gating of MthK, a prokaryotic potassium channel

Zadek B, Nimigean CM. J Gen Physiol. 2006 Jun;127(6):673-85. doi: 10.1085/jgp.200609534

2004

A cyclic nucleotide modulated prokaryotic K+ channel

Nimigean CM, Shane T, Miller C. J Gen Physiol. 2004 Sep;124(3):203-10. doi: 10.1085/jgp.200409133

2003

Electrostatic tuning of ion conductance in potassium channels

Nimigean CM, Chappie JS, Miller C. Biochemistry. 2003 Aug 12;42(31):9263-8. doi: 10.1021/bi0348720

2002

Slo1 tail domains, but not the Ca2+ bowl, are required for the beta 1 subunit to increase the apparent Ca2+ sensitivity of BK channels

Qian X, Nimigean CM, Niu X, Moss BL, Magleby KL. J Gen Physiol. 2002 Dec;120(6):829-43. doi: 10.1085/jgp.20028692

Na+ block and permeation in a K+ channel of known structure

Nimigean CM, Miller C. J Gen Physiol. 2002 Sep;120(3):323-35. doi: 10.1085/jgp.20028614

2000

Functional coupling of the beta(1) subunit to the large conductance Ca(2+)-activated K(+) channel in the absence of Ca(2+). Increased Ca(2+) sensitivity from a Ca(2+)-independent mechanism

Nimigean CM, Magleby KL. J Gen Physiol. 2000 Jun;115(6):719-36. doi: 10.1085/jgp.115.6.719

1999

Ca2+-dependent gating mechanisms for dSlo, a large-conductance Ca2+-activated K+ (BK) channel

Moss BL, Silberberg SD, Nimigean CM, Magleby KL. Biophys J. 1999 Jun;76(6):3099-117. doi: 10.1016/S0006-3495(99)77462-X

The beta subunit increases the Ca2+ sensitivity of large conductance Ca2+-activated potassium channels by retaining the gating in the bursting states

Nimigean CM, Magleby KL. J Gen Physiol. 1999 Mar;113(3):425-40. doi: 10.1085/jgp.113.3.425